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MAB5790-I

Sigma-Aldrich

Anti-DSD-1 Antibody, clone 473HD

clone 473HD, from rat

Sinónimos:

DSD-1-proteoglycan

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

473HD, monoclonal

species reactivity

mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

NP_001074775

UniProt accession no.

Q9WUT8

shipped in

wet ice

target post-translational modification

unmodified

General description

Anti-DSD-1 reacts with a glycosaminoglycan epitope selectively expressed on radial gliacytes of which a subpopulation shows stem cell properties. Cells isolated with this antibody from embryonic mouse tissue had multipotent characteristics and could turn into neurons, astrocytes and oligodendrocytes. The epitope is highly conserved and has been found in several vertebrates.

Immunogen

Purified tissue fraction corresponding to mouse DSD-1.

Application

Anti-DSD-1 Antibody, clone 473HD is an antibody against DSD-1, clone 473HD for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DSD-1 in mouse hippocampus, mouse cerebellum, and mouse embryo tissue.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DSD-1 in 10 µg of mouse brain tissue lysate.

Target description

~400 kDa observed

Linkage

Replaces: MAB5790

Physical form

Format: Purified
Purified rat monoclonal IgMκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Zaman Mirzadeh et al.
Nature metabolism, 1(2), 212-221 (2019-06-28)
In leptin-deficient ob/ob mice, obesity and diabetes are associated with abnormal development of neurocircuits in the hypothalamic arcuate nucleus (ARC)1, a critical brain area for energy and glucose homeostasis2,3. As this developmental defect can be remedied by systemic leptin administration

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