MAB1951Z

Anti-Integrin beta 1 (CD29) Antibody

CHEMICON^®, mouse monoclonal, P4G11

• Sinónimos: CD29, MAB1951
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• Nombre del producto: Anti-Integrin β1 Antibody, clone P4G11, azide free, clone P4G11, Chemicon^®, from mouse
• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: P4G11, monoclonal
• reactividad de especies: monkey, human, rabbit
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotipo: IgG1
• Nº de acceso NCBI: NM_002211.2; NM_033666.1; NM_033667.1; NM_033668.1; NM_033669.1; NM_133376.1
• Nº de acceso UniProt: P05556
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ITGB1(3688)

Descripción

Especificidad

Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.

Inmunógeno

Human IL-1beta activated endothelial cells

Aplicación

Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.

Forma física

Format: Purified

Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.

Almacenamiento y estabilidad

Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable