MAB1137Z
Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9
clone 47-9, Chemicon®, from mouse
Sinónimos:
CD220, MAB1137
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
47-9, monoclonal
reactividad de especies
human, pig, sheep, bovine
no debe reaccionar con
rat, mouse
fabricante / nombre comercial
Chemicon®
isotipo
IgG1
idoneidad
not suitable for Western blot
not suitable for immunoprecipitation
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... INSR(3643)
Especificidad
Recognizes a protein of 135kDa, identified as the alpha-subunit of insulin receptor (IR). Its epitope localizes in exon 7/8 and shows no cross-reaction with IGF-receptors {Soos, 1986}. Antibody superbly inhibits (>90%) insulin binding and BLOCKS insulin action. IR is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-a-a-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit is completely extracellular. The intracellular portion of the beta-subunit is a tyrosine-specific protein kinase with sequence homology to other such kinases. Activation of kinase is essential in signaling pathway whereby insulin regulates intracellular metabolism.
Inmunógeno
Epitope: alpha subunit, azide free
IM-9 lymphocytes followed by purified insulin receptor.
Aplicación
Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9 detects level of Insulin Receptor & has been published & validated.
Completely (>90%) Inhibits Insulin Binding: use antibody at 10nM
Antagonist: Blocks insulin action
Does not work in immunoprecipitation {Soos, 1986}.
Optimal working dilutions must be determined by end user.
Antagonist: Blocks insulin action
Does not work in immunoprecipitation {Soos, 1986}.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Forma física
Format: Purified
Protein G Purified
Protein G Purified mouse immunoglobulin in 10mM PBS, pH 7.4 contain no preservatives.
Almacenamiento y estabilidad
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análisis
Control
3T3 cells expressing transfected human insulin receptor
3T3 cells expressing transfected human insulin receptor
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Veronica Rivi et al.
Neuroendocrinology, 113(4), 406-422 (2022-10-13)
The Garcia effect, a solid learning paradigm, was used to investigate the molecular and behavioral effects induced by different lengths of fasting on the cognitive functions in the pond snail Lymnaea stagnalis, a valid model system. Three experimental groups were
Waldemar B Minich et al.
The Journal of clinical endocrinology and metabolism, 108(9), 2324-2329 (2023-03-05)
Severe insulin resistance (IR) in the presence of insulin receptor autoantibodies (InsR-aAb) is known as type B insulin resistance (TBIR). Considerable progress in therapy has been achieved, but diagnosis and monitoring of InsR-aAb remains a challenge. This work aimed to
Amer Youssef et al.
Endocrinology, 157(3), 1163-1174 (2016-01-14)
Placental mesenchymal stem cells (PMSCs) are readily available multipotent stem cells for potential use in regenerative therapies. For this purpose, PMSCs must be maintained in culture conditions that mimic the in vivo microenvironment. IGFs (IGF-1 and IGF-2) and oxygen tension
Carlos Godoy-Parejo et al.
Stem cells (Dayton, Ohio), 37(8), 1030-1041 (2019-04-26)
Insulin is present in most maintenance media for human embryonic stem cells (hESCs), but little is known about its essential role in the cell survival of individualized cells during passage. In this article, we show that insulin suppresses caspase cleavage
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico