MAB10750

Anti-LIMK1 Antibody, clone 40/00-1B11-1-1

clone 40/00-1B11-1-1, from rat

• Sinónimos: LIM domain kinase 1, LIM motif-containing protein kinase
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 40/00-1B11-1-1, monoclonal
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NM_010717.2
• Nº de acceso UniProt: P53668
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... LIMK1(3984)

Descripción

Descripción general

LIM Kinase 1 (LIMK1) is a protein kinase that plays an important role in cellular functions like cell motility and growth cone actin dynamics. LIMK1 contains one PDZ domain and two LIM domains which mediate protein to protein inteactions. It is widely expressed in the adult and fetal nervous system with the highest levels being found in the cerebral cortex. Mutations in LIMK1 have been associated with cardiovascular and musculo-skeletal abnormalities.

Especificidad

The antibody recognizes LIMK1.

Inmunógeno

Epitope: Unknown

GST-tagged recombinant protein corresponding to a region of mouse LIMK1.

Aplicación

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-LIMK1 Antibody, clone 40/00-1B11-1-1 is validated for use in WB, IP, IC, IH for the detection of LIMK1.

Calidad

Evaluated by Western Blot in PC3 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected LIMK1 on 10 µg of PC3 cell lysate.

Descripción de destino

~ 73 kDa

Forma física

Format: Purified

Protein G Purified

Purified in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
PC3 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable