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Nombre del producto
Immobilon®-FL PVDF Membrane, 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Quality Level
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, western blot: suitable
filter L × W
10 cm × 10 cm
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
General description
Application
Features and Benefits
- The first transfer membrane designed for fluorescence applications Extremely low background improves the sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- compatible with standard blocking agents and buffers
- Ideal for multiplexing and chemifluorescence detections
Legal Information
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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"Timeline of western blotting history. Timeless recipes and solutions for blotting success. Prepare and grow cell, media and sera with cultureware. Filter media with Stericup. ProteoExtract® kits for fractionation. CytoBuster™, BugBuster™ and CelLytic™ protein extraction reagents. Benzonase® nuclease. cOmplete™ and SIGMAFAST™ protease inhibitorsMaintain and preserve protein functionality following cell lysis using individual protease inhibitors and protease inhibitor cocktails. Amicon® filters for concentration and buffer exchange. PureProteome® magnetic beads for enrichment and purification. Recombinant protein purification affinity gels. Unlike methods that require careful aspiration to avoid sample loss, PureProteome® magnetic beads are isolated using a magnetic rack, for total removal of buffers and complete recovery with no sample dilution. TruPAGE™ precast gels Buffers (including Trizma®Tris base), solvents, prestained MW markers. Direct Detect® IR spectrometer for protein sample qualification. TruPAGE® precast gels are tear-resistant to reduce handling failures that commonly occur during gel electrophoresis and downstream processing. Range of Immobilon® PVDF transfer membranes. Western-validated antibodies. SNAP i.d.® 2.0 protein detection system. Immunodetection reagents. Blot efficiently using our Western blotting reagents, including Bløk® noise-cancelling reagents, Luminata™ Western HRP substrates, Immunoreaction Enhancer Reagents, and ReBlot™ Plus antibody stripping kits, all carefully optimized for your success."