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Merck

IPFL10100

Immobilon® -FL PVDF Membrane

10 sheets, 10 cm x 10 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

Sinónimos:

Western blotting membrane, blotting membrane, transfer membrane

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UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
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Nombre del producto

Immobilon®-FL PVDF Membrane, 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Quality Level

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

10 cm × 10 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

General description

The Immobilon®-FL transfer membrane is hydrophobic polyvinylidene fluoride (PVDF) microporous membrane used for the transfer of proteins from a variety of gel matrices. This transfer membrane offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. The Immobilon®-FL membrane has a nominal pore size of 0.45 μm and offers optimal blotting for proteins with molecular weights greater than 20,000 Daltons.

Application

Immobilon®-FL membrane has been used in western blotting. It is also suitable for use in standard chemiluminescent or chromogenic detection.

Features and Benefits

Immobilon®-FL Membrane:
  • The first transfer membrane designed for fluorescence applications Extremely low background improves the sensitivity of all fluorescence detection protocols
  • Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
  • compatible with standard blocking agents and buffers
  • Ideal for multiplexing and chemifluorescence detections

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificados de análisis (COA)

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Contenido relacionado

"Timeline of western blotting history. Timeless recipes and solutions for blotting success. Prepare and grow cell, media and sera with cultureware. Filter media with Stericup. ProteoExtract® kits for fractionation. CytoBuster™, BugBuster™ and CelLytic™ protein extraction reagents. Benzonase® nuclease. cOmplete™ and SIGMAFAST™ protease inhibitorsMaintain and preserve protein functionality following cell lysis using individual protease inhibitors and protease inhibitor cocktails. Amicon® filters for concentration and buffer exchange. PureProteome® magnetic beads for enrichment and purification. Recombinant protein purification affinity gels. Unlike methods that require careful aspiration to avoid sample loss, PureProteome® magnetic beads are isolated using a magnetic rack, for total removal of buffers and complete recovery with no sample dilution. TruPAGE™ precast gels Buffers (including Trizma®Tris base), solvents, prestained MW markers. Direct Detect® IR spectrometer for protein sample qualification. TruPAGE® precast gels are tear-resistant to reduce handling failures that commonly occur during gel electrophoresis and downstream processing. Range of Immobilon® PVDF transfer membranes. Western-validated antibodies. SNAP i.d.® 2.0 protein detection system. Immunodetection reagents. Blot efficiently using our Western blotting reagents, including Bløk® noise-cancelling reagents, Luminata™ Western HRP substrates, Immunoreaction Enhancer Reagents, and ReBlot™ Plus antibody stripping kits, all carefully optimized for your success."

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