Anti-Cytokeratin 14 Antibody, clone LL002 is an antibody against Cytokeratin 14 for use in WB, IH(P).
Suitable for staining formalin fixed wax sections or frozen tissues. In order to obtain 100% consistency in staining it is considered essential to either microwave treat fixed tissue sections or alternatively use the more recently recommended high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use with CBL 197 is neutral buffered formalin.
Western blotting
Optimal working dilutions must be determined by the end user.
Quality
Tested
Physical form
Format: Purified
The monoclonal is presented at a concentration of 100 μg/mL in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Other Notes
Please refer to lot specific datasheet.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Methods in molecular biology (Clifton, N.J.), 2749, 25-38 (2023-12-22)
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