Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Especificidad
Reacts with human lambda light chains. Absorbed for human myeloma proteins with kappa light chains.
The antibody reacts with human lambda light chains as demonstrated by ELISA and flow cytometry.
Inmunógeno
Epitope: Ig lambda light chain
Prepared from pooled antisera from goats hyperimmunized with human myeloma proteins with lambda light chains.
Aplicación
ELISA: 1:4,000-1:8,000.
Optimal working dilutions must be determined by the end user.
Goat anti-Human Ig λ chain Antibody, HRP conjugate detects level of Human Ig λ chain & has been published & validated for use in ELISA.
Research Category Secondary & Control Antibodies
Research Sub Category Fragment Specific Secondary Antibodies
Forma física
Affinity Chromatography
Purified by affinity chromatography on pooled human Igs with lambda light chains covalently linked to agarose. Liquid in 50% glycerol/50% PBS, pH 7.4.
Almacenamiento y estabilidad
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
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When constructing isogenic recombinant IgM-IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired with κ light chains, IgM yields were
The Journal of clinical investigation, 134(8) (2024-03-12)
BACKGROUNDWeakly virulent environmental mycobacteria (EM) can cause severe disease in HLA-DRB1*15:02 or 16:02 adults harboring neutralizing anti-IFN-γ autoantibodies (nAIGAs). The overall prevalence of nAIGAs in the general population is unknown, as are the penetrance of nAIGAs in HLA-DRB1*15:02 or 16:02
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