ABS232

Anti-MIXL1 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Homeobox protein MIXL1, Homeodomain protein MIX, hMix, MIX1 homeobox-like protein 1, Mix.1 homeobox-like protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): primate (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_114150
• Nº de acceso UniProt: Q9H2W2
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MIXL1(83881)

Descripción

Descripción general

MIXL1 is also known as Homeobox protein MIXL1, Homeodomain protein MIX (hMix), MIX1 homeobox-like protein 1, and Mix.1 homeobox-like protein. MIXL1 is a transcription factor with roles in axial mesendoderm morphogenesis, endoderm formation, blood cell differentiation, and morphogenesis of the heart and the gut during embryogenesis. MIXL1 has been shown to negatively regulate brachyury expression. MIXL1 is expressed mainly in progenitors and secondary lymph tissues, immature B- and T-lymphoid cells during normal hematopoiesis, and is also present in differentiating embryonic stem cells.

Especificidad

Other homologies: Mouse and Rat (85% sequence homology).

This antibody recognizes the homeobox DNA binding domain of MIXL1.

Inmunógeno

Epitope: Homeobox DNA binding domain

KLH-conjugated linear peptide corresponding to the homeobox DNA binding domain of human MIXL1.

Aplicación

Anti-MIXL1 Antibody is a highly specific rabbit polyclonal antibody, that targets Homeobox protein MIXL1 & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected MIXL1 in H9 embryoid body cells. This protein was observed in H9 embryoid bodies (Lee, M. R., et al. (2010). Stem Cells. 28:1550-1559). MIXL1 was also localized in the nucleus of COS-7 and mouse ES cells (Mossman, A. K., et al. (2005). Stem Cells and Development. 14:656-663).

Research Category
Signaling

Research Sub Category
Developmental Signaling

Calidad

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MIXL1 in 10 µg of Jurkat cell lysate.

Descripción de destino

~36 kDa observed

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Jurkat cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable