ABE1044
Anti-HSF 1 Antibody
serum, from rabbit
Sinónimos:
Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
human, mouse
técnicas
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... HSF1(3297)
Descripción general
HSF1, also known as Heat shock factor protein 1 (HSF-1) or Heat shock transcription factor 1 (HSTF-1), and encoded by the gene HSF1/HSTF1, is a DNA binding transcription factor that binds heat shock promoter elements (HSE’s) and activates transcription. In higher eukaryotes, HSF1 is unable to bind to HSE regions unless the cells are heat shocked. HSF1 is normally a monomer that interacts with HSP90 and other chaperones in a large complex in the cytoplasm. Upon heat shock HSF1 is released from the complex, dimerizes, and is transported into the nucleus. HSF1 is localized to the cytoplasm during normal growth the protein localizes to the nucleus upon heat shock. HSF1 is part of the cellular stress response, and HSF1 is known to interact with many other stress- induced proteins including NCOA6, SYMPK, RALBP1, HSF2, CEBPB, HSPA1A, Heat shock protein 90kDa alpha and HSPA.
Inmunógeno
Recombinant protein corresponding to mouse HSF 1.
Aplicación
Anti-HSF 1 antibody is an antibody against HSF 1 for use in western blotting, IP & ChIP.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HEK293 cell lysates transfected with wild type or mutant hRP1-HA (Fujimoto, M., et al. (2012). Mol Cell. 48(2):182-194.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from hHSF1 transfected MEF cell lysate (Fujimoto, M., et al. (2010). Mol Cell Biol. 21(1):106-116.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from wild type and HSF2-null primary MEF cell lysates (Shinkawa, T., et al. (2011). Mol Cell Biol. 22(19):3571-3583.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HeLa and MEF cell lysates which were either untreated or heat shock treated (Prof. A. Nakai, Yamaguchi University School of Medicine).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from hHSF1 transfected MEF cell lysate (Fujimoto, M., et al. (2010). Mol Cell Biol. 21(1):106-116.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from wild type and HSF2-null primary MEF cell lysates (Shinkawa, T., et al. (2011). Mol Cell Biol. 22(19):3571-3583.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HeLa and MEF cell lysates which were either untreated or heat shock treated (Prof. A. Nakai, Yamaguchi University School of Medicine).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Calidad
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting: A 1:1,000 dilution of this antibody detected HSF 1 in 10 µg of HeLa cell lysate.
Western Blotting: A 1:1,000 dilution of this antibody detected HSF 1 in 10 µg of HeLa cell lysate.
Descripción de destino
~75 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Forma física
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Ryosuke Takii et al.
The EMBO journal, 38(24), e102566-e102566 (2019-10-28)
The recruitment of RNA polymerase II (Pol II) to core promoters is highly regulated during rapid induction of genes. In response to heat shock, heat shock transcription factor 1 (HSF1) is activated and occupies heat shock gene promoters. Promoter-bound HSF1
Ke Tan et al.
Nature communications, 6, 6580-6580 (2015-03-13)
Heat-shock response is an adaptive response to proteotoxic stresses including heat shock, and is regulated by heat-shock factor 1 (HSF1) in mammals. Proteotoxic stresses challenge all subcellular compartments including the mitochondria. Therefore, there must be close connections between mitochondrial signals
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The mitochondrial unfolded protein response (UPRmt ) is characterized by the transcriptional induction of mitochondrial chaperone and protease genes in response to impaired mitochondrial proteostasis and is regulated by ATF5 and CHOP in mammalian cells. However, the detailed mechanisms underlying
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Molecular and cellular biology, 38(18) (2018-06-27)
Activation of the adaptive response to cellular stress orchestrated by heat shock factor 1 (HSF1), which is an evolutionarily conserved transcriptional regulator of chaperone response and cellular bioenergetics in diverse model systems, is a central feature of organismal defense from
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