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574795

Sigma-Aldrich

TBE Buffer, 10X, Molecular Biology Grade

A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.

Sinónimos:

Tris-Borate-EDTA Buffer

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

Molecular Biology

Formulario

liquid

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

actividad extraña

DNase, RNase, protease, none detected

Condiciones de envío

ambient

temp. de almacenamiento

15-25°C

Descripción general

A 10X concentrate that can be diluted to a 1X solution containing 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH ~8.3.
Tris, borate, and ethylenediaminetetraacetic acid (TBE) buffer is used to perform agarose gel electrophoresis of DNA and RNA. It consists of a weak acid that exists in neutral and anionic forms (e.g., COOH and COO- species). Tris is a weak base, which occurs in either neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions maintain a low conductivity medium, transmit the electrical current, and buffer the pH. Ethylenediaminetetraacetic (EDTA) although not essential is added as a preventative since it chelates Mg2+ ions and inactivates potential DNA nucleases. TBE allows an effective resolution of DNA fragments and slightly improves the separation of smaller fragments.[1] It also stabilizes nucleic acids against enzymatic degradation.[2]

Aplicación

TBE Buffer, 10X, Molecular Biology Grade-Calbiochem has been used as a component of polyacrylamide sequencing gels for DNA electrophoresis using amplified fragment length polymorphism (AFLP).[3]

Advertencia

Toxicity: Irritant (B)

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H360FD

Clasificaciones de peligro

Repr. 1B

Código de clase de almacenamiento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Marjan Sadat Hosseini et al.
Phytochemistry, 183, 112629-112629 (2021-01-31)
Licorice (Glycyrrhiza glabra L.) is a medicinal plant species valued in many countries in Asia and Europe for its phytochemical characteristics. Licorice biodiversity is becoming threatened nowadays in Iran due to increasing demand and a drastic decline of its natural
Optical properties of buffers and cell culture media for optofluidic and sensing applications
Hoang VT, et al.
Applied Sciences, 9(6), 1145-1145 (2019)
Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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