529625

Polyphosphoinositide-Binding Peptide, PBP10

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin.

• Sinónimos: Polyphosphoinositide-Binding Peptide, PBP10, RhoB-GS _160-169, Rhodamine B-QRLFQVKGRR-OH, RhoB-GS_160-169, Rhodamine B-QRLFQVKGRR-OH
• Fórmula empírica (notación de Hill): C₈₄H₁₂₇N₂₄O₁₅
• Número de CAS: 794466-45-8
• Peso molecular: 1713.06
• UNSPSC Code: 12352211
• NACRES: NA.77

Propiedades

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: pink-red
• solubility: DMSO: 2 mg/mL
• fluorescence: λ_ex 565 nm; λ_em 590 nm
• shipped in: ambient
• storage temp.: −20°C

Descripción

General description

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelet without significantly affecting intracellular Ca²⁺ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP₂) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs.

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelets without significantly affecting intracellular Ca²⁺ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP₂) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Excitation max.: ~565 nm; emission max.: ~590 nm.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Specifically binds to polyphosphoinositide (PPI)

Product does not compete with ATP.

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Rho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Bucki, R., et al. 2001. Biochemistry40, 15752
Cunningham, C.C., et al. 2001. J. Biol. Chem.276, 43390.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable