499260
Osteopontin, His•Tag®, Mouse, Recombinant, Mouse
Sinónimos:
rmOPN
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.45
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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Ensayo
≥95% (SDS-PAGE)
Nivel de calidad
Formulario
lyophilized
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
impurezas
≤100 pg/μg Endotoxins (pg/μg rmOPN)
Condiciones de envío
ambient
temp. de almacenamiento
−70°C
Descripción general
M.W. 65,000 and 30,000 under reducing conditions.
Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.
Acciones bioquímicas o fisiológicas
~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10⁵ cells/well
Advertencia
Toxicity: Standard Handling (A)
Forma física
Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.
Reconstitución
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
Reconstitute to ≥100 µg/ml with sterile PBS.
Otras notas
Xie, Y., et al. 2001. Kidney Int.60, 1645.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Kristen Johnson et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 18(6), 994-1004 (2003-06-24)
Osteopontin and PP(i) both suppress hydroxyapatite deposition. Extracellular PP(i) deficiency causes spontaneous hypercalcification, yet unchallenged osteopontin knockout mice have only subtle mineralization abnormalities. We report that extracellular PP(i) deficiency promotes osteopontin deficiency and correction of osteopontin deficiency prevents hypercalcification, suggesting
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