496260

Oncostatin M, Human, Recombinant, E. coli

• Sinónimos: Oncostatin M, Human, Recombinant, E. coli
• Número de CAS: 106956-32-5
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 100
• Ensayo: ≥97% (SDS-PAGE)
• Formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• impurezas: ≤1 EU/μg Endotoxin (EU/μg rhOSM)
• Condiciones de envío: ambient
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Recombinant, human oncostatin M expressed in E. coli. Pleiotropic cytokine which inhibits growth of A375 melanoma cells and mouse M1 myeloid leukemic cells. Enhances the growth of both endothelial and hematopoietic cells. Has also been reported to have growth stimulatory activities on normal fibroblasts, AIDS-Kaposis sarcoma cells, and a human erythroleukemic cell line, TF-1.

Recombinant, human oncostatin M expressed in E. coli. Pleiotropic cytokine which inhibits growth of A375 melanoma cells and mouse M1 myeloid leukemic cells. Has also been reported to have growth stimulatory activities on normal fibroblasts, AIDS-Kaposi′s sarcoma cells, and human erythroleukemia cell line, TF-1.

Acciones bioquímicas o fisiológicas

ED₅₀ = 0.15-0.30 ng/ml as measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg rhOSM.

Reconstitución

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a final concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% BSA or HSA.

Otras notas

Boileau, C., et al. 2000. J. Immunol.164, 5713.
Miyajima, A., et al. 2000. Cytokine Growth Factor Rev.11, 177.
Linsley, P.S., et al. 1990. Mol. Cell. Biol.10, 1882.
Kitamura, T., et al. 1989. J. Cell Physiol.140, 323.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3