492028

NRG1-β1/HRG1-β1 ECD, Human, Recombinant

• Código UNSPSC: 12352203
• NACRES: NA.77

Propiedades

• Ensayo: ≥90% (SDS-PAGE)
• Nivel de calidad: 100
• Formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• impurezas: ≤1.0 EU/μg protein Endotoxin
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Recombinant, human neuregulin 1-β1/heregulin 1-β1 extracellular domain (NRG1-beta1/HRG1-beta1 ECD) consisting of amino acids 2-246 and expressed in E. coli. All NRG1 isoforms contain an EGF-like domain (β-splice variant) that is used to directly bind to the ErbB3 or ErbB4 receptor tyrosine kinases.

Acciones bioquímicas o fisiológicas

ED₅₀ = 2.5-12.5 ng/ml as measured in a serum-free proliferation assay using MCF7 human breast adenocarcinoma cells.

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from 0.2 µm filtered acetonitrile, TFA, BSA.

Reconstitución

Following reconstitution refrigerate (4°C) for short term-storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute 50 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Otras notas

Buonanno, A., and G.D Fischbach 2001. Curr. Opin. Neurobiol.11, 287.
Adlkofer, K. and C. Lai 2000. Glia29, 104.
Garratt, A.N. et al., 2000. BioEssays22, 987.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3