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Merck
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Documentos clave

324506

Millipore

EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated

Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.

Sinónimos:

EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated, Ethylenediaminetetraacetic Acid Trisodium Salt

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About This Item

Fórmula empírica (notación de Hill):
C10H13N2Na3O8 · 3H2O
Número de CAS:
Peso molecular:
412.23
Número MDL:
Código UNSPSC:
12352107
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

grado

Molecular Biology

Nivel de calidad

Formulario

liquid

idoneidad de la reacción

reagent type: chelator

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

pH

8

Condiciones de envío

ambient

temp. de almacenamiento

15-25°C

cadena SMILES

[Na+].[Na+].[Na+].N(CCN(CC(=O)[O-])CC(=O)O)(CC(=O)[O-])CC(=O)[O-].O.O.O

InChI

1S/C10H16N2O8.3Na.3H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;3*1H2/q;3*+1;;;/p-3

Clave InChI

FXNQQEVEDZAAJM-UHFFFAOYSA-K

Descripción general

Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.

Aplicación

  • Comparing extraction method efficiency for high-throughput palaeoproteomic bone species identification.: This research compares various extraction methods for palaeoproteomic analysis, including those utilizing EDTA, to identify bone species efficiently. The study underscores the importance of EDTA in enhancing protein extraction for proteomic studies (Mylopotamitaki et al., 2023).

  • Deep coverage and quantification of the bone proteome provides enhanced opportunities for new discoveries in skeletal biology and disease.: The use of EDTA in the preparation and extraction of bone samples for proteomic analysis is discussed, emphasizing its role in achieving deep proteomic coverage and facilitating new discoveries in skeletal biology (Rose et al., 2023).

Advertencia

Toxicity: Standard Handling (A)

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Health hazardCorrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Dam. 1 - STOT RE 2 Inhalation

Órganos de actuación

Respiratory Tract

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, Is the DEPC innactivated in this solution ? I have some leftover and I am wondering if it would be suitable for use in a cell culture buffer context ? Thank you in advance for your support, Kind regards, Coline

    1 answer
    1. Yes, DEPC was inactivated. Note that one of the break down products of DEPC is ethanol. DEPC-treated solutions are typically not used in cell culture applications and may be cytotoxic.

      Helpful?

  4. Dear all, Is the EDTA 0.5 M solution prepared with before DEPC treated H2O, or, is it prepared together with DEPC and then autoclaved? Many thanks for your answer! Best, Julia

    1 answer
    1. The solution is prepared with DEPC-treated H2O.

      Helpful?

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