217695

Cdk1 Inhibitor - CAS 220749-41-7 - Calbiochem

The Cdk1 Inhibitor, also referenced under CAS 220749-41-7, controls the biological activity of Cdk1. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Sinónimos: Cdk1 Inhibitor - CAS 220749-41-7 - Calbiochem, 3-(2-Chloro-3-indolylmethylene)-1,3-dihydroindol-2-one
• Fórmula empírica (notación de Hill): C₁₇H₁₁ClN₂O
• Número de CAS: 220749-41-7
• Peso molecular: 294.74
• UNSPSC Code: 12352200

Propiedades

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: orange
• solubility: DMSO: 200 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

A cell-permeable indolylmethylene-2-indolinone derivative that exhibits potent anti-proliferative properties (IC₅₀ = 2 µM HeLa cells). Acts as a selective, reversible and ATP-competitive inhibitor of Cdk1/cyclin B (IC₅₀ = 5.8 µM). Also inhibits Cdk5 with a IC₅₀ = 25 µM. Binds to the ATP pocket on the Cdk1 active site. Does not affect the activity of GSK-3β even at 100 µM concentrations.

A cell-permeable indolylmethylene-2-indolinone derivative that exhibits potent anti-proliferative properties (IC₅₀ = 2 µM in HeLa cells). Acts as a selective, reversible and ATP-competitive inhibitor of cyclin-dependent kinase-1/cyclin B (IC₅₀ = 5.8 µM for Cdk1 and 25 µM for Cdk5). Does not affect the activity of GSK-3β even at 100 µM concentrations. Binds to the ATP pocket in the Cdk1 active site.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Cdk1

Product competes with ATP.

Reversible: yes

Target IC₅₀: 2 µM for anti-proliferative properties in HeLa cells; 5.8 µM against Cdk1/cyclin B

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Andreani, A., et al. 2000. Anticancer Drug Des.15, 447.
Andreani, A., et al. 1996. Anticancer Res.16, 3585.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10-13 - German Storage Class 10 to 13