14-926-M

IKKε Protein, active, 10 µg

Active, N-terminal FLAG & GST-tagged, recombinant, human IKKε, full length. For use in Kinase Assays.

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Propiedades

• biological source: human
• Quality Level: 300
• recombinant: expressed in baculovirus infected Sf21 cells
• mol wt: M_w 109 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_001193321.1
• UniProt accession no.: Q14164

Descripción

General description

IKBKE is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.

Product Source: N-terminal FLAG and GST-tagged, recombinant, human IKK full length, expressed by baculovirus in Sf21 insect cells.

Application

Research Category
Signaling

Biochem/physiol Actions

Protein Target: IKKe

Target Sub-Family: Misc

Packaging

Also available in 250μg size as catalog # 14-926M and in bulk as catalog # 14-926-K.

Quality

Routinely evaluated by phosphorylation of casein.

Physical form

50mM Tris/HCl pH7.5, 150mM NaCl, 10mM β-glycerophosphate, 1mM sodium orthovanadate, 1mM NaF, 28% v/v glycerol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol

Purified by immunoaffinity chromatography.

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable