1.08380

Cloruro de 2,3,5-trifeniltetrazolio

Color indicator. Supplement in culture media for the microbiological analysis of food and water samples

• Sinónimos: Rojo de tetrazolio, TPTZ, TTZ
• Fórmula empírica (notación de Hill): C₁₉H₁₅ClN₄
• Número de CAS: 298-96-4
• Peso molecular: 334.80
• Beilstein: 3923356
• Número MDL: MFCD00011963
• Código UNSPSC: 41106212
• NACRES: NA.74

Propiedades

• Nivel de calidad: 200
• Ensayo: ≥98% (calculated on dried substance, perchloric acid titration)
• Formulario: solid
• pH: 3.7 (20 °C, 10 g/L in H₂O)
• mp: 243 °C (decomposition)
• solubilidad: 150 g/L
• densidad aparente: 230 kg/m³
• aplicaciones: microbiology
• temp. de almacenamiento: 2-30°C
• cadena SMILES: [Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4
• InChI: 1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1
• Clave InChI: PKDBCJSWQUOKDO-UHFFFAOYSA-M

Descripción

Descripción general

2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to formation of formazan. Live microorganisms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells.

Aplicación

2,3,5-triphenyltetrazolium chloride (TTC) is an indicator recommended for the microbiological analysis of food.

Nota de análisis

Assay (perchloric acid titration, calculated on dried substance): ≥ 98 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ_max. (water): 245 - 248 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0,01 g/l; water; calculated on dried substance): ≥ 770
TLC-Test: passes test
Loss on drying (70 °C; ≤ 1 hPa): ≤ 0.5 %
Suitability for testing the germinability of seeds: passes test
Suitability for microbiology: passes test

Información de seguridad

• Pictogramas: GHS02,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H228 - H315 - H319
• Consejos de prudencia: P210 - P240 - P241 - P264 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2
• Código de clase de almacenamiento: 4.1B - Flammable solid hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3