07-507
Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody
Upstate®, from rabbit
Sinónimos:
Anti-Anti-BMK1, Anti-Anti-ERK4, Anti-Anti-ERK5, Anti-Anti-PRKM7
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, bovine, human
manufacturer/tradename
Upstate®
technique(s)
inhibition assay: suitable (peptide)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr218/pTyr220)
Gene Information
bovine ... Mapk7(537703)
human ... MAPK7(5598)
mouse ... Mapk7(23939)
rat ... Mapk7(114509)
Specificity
Recognizes phospho-BMK1/Erk5 (Thr218, Tyr220).
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5
Application
Peptide Inhibition Analysis:
HeLa cell lysate, HeLa cell lysate+ non-phosphorylated protein, and HeLa cell lysate + phosphorylated peptide were probed with Cat. No. 07-507, Anti-phospho-BMK 1/ERK5 (Thr218/Tyr220) (1µg/mL(1:1000) dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system.
Arrow indicates BMK1/ERK5 (~115 kDa) on HeLa cell lysate & HeLa cell lysate + non-phosphorylated peptide. No band is present on the HeLa cell lysate + phosphorylated peptide indicating successful peptide inhibition of the antibody.
HeLa cell lysate, HeLa cell lysate+ non-phosphorylated protein, and HeLa cell lysate + phosphorylated peptide were probed with Cat. No. 07-507, Anti-phospho-BMK 1/ERK5 (Thr218/Tyr220) (1µg/mL(1:1000) dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system.
Arrow indicates BMK1/ERK5 (~115 kDa) on HeLa cell lysate & HeLa cell lysate + non-phosphorylated peptide. No band is present on the HeLa cell lysate + phosphorylated peptide indicating successful peptide inhibition of the antibody.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
This Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody is validated for use in WB for the detection of phospho-BMK1/Erk5 (Thr218/Tyr220).
Quality
Evaluated by Western Blotting/Peptide Inhibition Analysis in HeLa cell lysate.
Western Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected phospho-BMK1/Erk5 (Thr218/Tyr220) in HeLa cell lysate.
Western Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected phospho-BMK1/Erk5 (Thr218/Tyr220) in HeLa cell lysate.
Target description
110-115kDa
Physical form
Affinity purified immunoglobulin in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at 2-8°C from date of shipment.
Analysis Note
Control
Sorbitol-treated HeLa cells
Sorbitol-treated HeLa cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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