05-1307-AF488

Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor^™ 488 conjugate

clone Apu2, from rabbit, ALEXA FLUOR^™ 488

• Sinónimos: Polyubiquitin-B, Ubiquitin, Lys48-Specific, Ubiquitin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: Apu2, monoclonal
• species reactivity: rat, mouse, human
• species reactivity (predicted by homology): rabbit (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable
• isotype: IgG
• NCBI accession no.: NP_001268645.1
• UniProt accession no.: P0CG47
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... UBB(7314) ; mouse ... Ubg(107729)

Descripción

General description

Polyubiquitin-B (UniProt P0CG47) is encoded by the UBB gene (Gene ID 7314) in human. Ubiqutin (Ub) is initially produced as a 229-a.a. precursor protein whose C-terminus Cys229 is post-translationally removed, followed by cleavages between a.a. 76 and 77, as well as between a.a. 152 and 153 to yield three idential 76-amino acid Ub molecules. Ub is involved in one of the most common post-translational modifications (PTMs) of cellular proteins, where Ub is linked covalently via its carboxyl terminus to usually Lys residues in target proteins. A given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyubiquitin chain, ubiquitin molecules can be linked through one of the seven ubiquitin Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). An Ub chain with a single linkage type is referred to as homotypic, whereas an heterotypic chain contains mixed linkages within the same chain, which can lead to branched/forked (as a result of one Ub linked to two or more Ubs) or non-branched Ub chains. Lys48-linked chains mark proteins for proteasomal degradation, while Lys63-linked chains regulate the activity/function of cellular proteins.

Specificity

Based on sequence homology, broad species cross-reactivity is expected.

Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.

Immunogen

Epitope: Lys48 ubiquitination site

Immunogen used was human di-ubiquitin linked at Lys48.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ubiquitin in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-1307) is shown to be suitable also for cell funtion assay, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, Western blotting applications.

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor^™ 488 conjugate is validated for use in Immunocytochemistry for the detection of Ubiquitin.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ubiquitin in HeLa cells.

Target description

~17 kDa observed

Physical form

Protein A purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable