05-1064

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2

clone 5C9.2, from mouse

• Sinónimos: Transcription factor ISGF-3 components p91/p84, signal transducer and activator of transcription 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 5C9.2, monoclonal
• reactividad de especies: rat, mouse, human
• técnicas: western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_009330
• Nº de acceso UniProt: P42224
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pTyr701)
• Información sobre el gen: human ... STAT1(6772) ; mouse ... Stat1(20846)

Descripción

Descripción general

STATs (Signal-Transducing Activators of Transcription) are the principle substrates of JAK kinases, and mediators of cytokine signaling. STAT proteins have SH2 domains, and once phosphorylated by JAK kinases, STATs dimerize in a head-to-tail fashion, through their SH2 domains. STAT1 is a critical mediator of IFN signaling, STAT4 is specific to IL-12, STAT6 is activated primarily by IL-4 and IL-13, while STAT5 A and B appear to be most important to the function of growth hormone and prolactin. STAT3 is involved in multiple signaling pathways, and mice deficient in it are not viable. In addition to tyrosine phosphorylation, many STAT proteins are targets of Ser/Thr kinases, and those phosphorylation events potentiate STAT-induced transcription. The phosphorylation state of STATs can be monitored by phosphorylation state-specific antibodies.

Especificidad

This antibody recognizes phosphorylated STAT1 at tyrosine 701.

Inmunógeno

Epitope: Phosphorylated Tyr701

KLH-conjugated linear peptide corresponding to the region around phosphorylated tyrosine 701.

Aplicación

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 detects level of Phospho-Stat1 (Tyr701) & has been published & validated for use in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Calidad

Routinely evaluated by western blot on EGF treated and untreated A431 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this lot was used to detect STAT1 phosphorylated at Tyr701 in EGF treated and untreated A431 cell lysates.

Descripción de destino

88-92 kDa

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS and 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8ºC from date of receipt.

Nota de análisis

Control
EGF treated and untreated A431 cell lysates, PDGF treated and untreated NIH/3T3 cell lysates, pervanadate treated and untreated PC12 cell lysates.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable