Cap B solution, containing tetrahydrofuran (THF), pyridine and N-methylimidazole (NMI), is mixed in situ with Cap A solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.Tetrahydrofurane has been replaced by acetonitrile in ÄKTA Cap B1 and B2.Cap B is configured for ÄKTA formulation.
Nota de preparación
Acetonitrile / Pyridine 4/6 (v/v)
Otras notas
Cap B2 solution, containing acetonitrile (ACN) and pyridine, is mixed in situ with Cap A and Cap B1 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.
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