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  • Simultaneous determination of the colorants sunset yellow FCF and quinoline yellow by solid-phase spectrophotometry using partial least squares multivariate calibration.

Simultaneous determination of the colorants sunset yellow FCF and quinoline yellow by solid-phase spectrophotometry using partial least squares multivariate calibration.

The Analyst (1997-04-01)
L F Capitán-Vallvey, M D Fernández, I de Orbe, J L Vilchez, R Avidad
ABSTRACT

A method for the simultaneous determination of the colorants Sunset Yellow FCF and Quinoline Yellow using solid-phase spectrophotometry is proposed. The colorants were isolated in Sephadex DEAE A-25 gel at pH 5.0, the gel-colorants system was packed in a 1 mm silica cell and spectra were recorded between 400 and 600 nm against a blank. Statistical results were obtained by partial least squares (PLS) multivariate calibration. The optimized matrix by using the PLS-2 method enables the determination of the colorants in artificial mixtures and commercial soft drinks.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Quinoline Yellow, for microscopy (Hist.), mixture of mono- and disulfonic acid sodium salt
Sigma-Aldrich
Quinoline Yellow, Mixture of the mono- and disulfonic acids of Quinoline Yellow
Sigma-Aldrich
4-Ethylphenol, ≥97.0% (GC)