814007

TSKgel^® Phenyl-5PW RP (10 µm) HPLC Columns

L x I.D. 7.5 cm × 8 mm, phenyl phase, octadecyl

• UNSPSC Code: 41115711

Properties

• product name: TSKgel^® Phenyl-5PW RP HPLC Column, phase phenyl, octadecyl, L × I.D. 7.5 cm × 8 mm, 10 μm particle size
• material: glass
• product line: TSKgel^®
• manufacturer/tradename: Tosoh 14007
• extent of labeling: 1 meq/mL ligand density (estimate)
• parameter: no limitation salt concentration; 0-100% organic solvent; 2.5 mL/min flow rate; 20 bar max. pressure; 4-60 °C temperature
• technique(s): HPLC: suitable
• L × I.D.: 7.5 cm × 8 mm
• matrix: hydroxylated methacrylic polymer
• matrix active group: phenyl phase, octadecyl
• particle size: 10 μm
• operating pH: 2-12
• separation technique: reversed phase

Description

General description

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of strong basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel^® columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

• Octadecyl-2PW particles have 125 A pores
• Octadecyl-4PW particles have 500 Apores
• Phenyl-5PW RP particles have 1000 A pores
• Octadecyl-NPR particles are nonporous

Physical form

Shipped in 30% methanol - 70% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation