It is a good antibody for Western Blot for both specimen of human and mouse. But it often shows signals medium intensity, not strong enough.
V8137
Anti-V5 antibody produced in rabbit
Anti-V5 antibody produced in rabbit
1 of 1 reviewers received a sample product or took part in a promotion
IgG fraction of antiserum
Synonym(s):
Rabbit Anti-V5
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0.2 MG
MXP 11,522.00
About This Item
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
technique(s)
immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells
immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells
western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.
Specificity
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Application
Anti-V5 antibody produced in rabbit has been used in:
- immunocytochemistry
- immunoprecipitation
- western blot
Physical form
Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.
Storage and Stability
Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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