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form
solid
Quality Level
ε (extinction coefficient)
115000 at 593 nm
absorbance ratio
A280/593 nm 0.08
fluorescence
λex 532 or 561 nm (Flow cytometry laser line)
λex 593 nm; λem 614 nm
λex 594 nm (Microscopy laser line)
suitability
suitable for fluorescence
storage temp.
−20°C
General description
CF™594 is a deep red fluorescent dye spectrally similar to Alexa Fluor™ 594 and Texas Red® dye. When conjugated to proteins, CF™594 is significantly brighter than Alexa Fluor™ 594 due to its high quantum yield and exceptional water solubility. CF™594 also has excellent photostability, making it ideal for demanding applications such as confocal microscopy and single molecule imaging. These properties make CF™594 the best deep-red dye for labeling proteins and nucleic acids. The dye is particularly useful in combination with our blue fluorescent CF™350, green fluorescent CF™488A and far red CF™647 for multi-color imaging.
Features and Benefits
1. Yields the brightest antibody conjugates among spectrally similar dyes; 2. Extremely photostable; 3. Highly water-soluble
Preparation Note
Protect from light
Legal Information
This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.
Texas Red is a registered trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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