SAB4200742

Anti-c-Myc-Peroxidase antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

• Synonym(s): Anti-MRTL, Anti-Myc, Anti-bHLHe39, Anti-v-myc avian myelocytomatosis viral oncogene homolog
• UNSPSC Code: 12352203
• NACRES: NA.56

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 9E10, monoclonal
• form: lyophilized powder
• species reactivity: human
• packaging: vial of 100 μL
• concentration: ~2 mg/mL
• technique(s): immunoblotting: 1:250-1:500 using lysate of HEK-293T cells over expressing c-Myc fusion protein; immunohistochemistry: suitable
• isotype: IgG1
• UniProt accession no.: P01106
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYC(4609)

Description

General description

Monoclonal Anti-c-Myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with synthetic peptide of the human p62c-myc protein, conjugated to KLH. c-Myc is a proto-oncogene localized on long arm of human chromosome 8. The encoded protein contains an N-terminal transactivation domain. The human c-Myc proto-oncogene is the cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses.

Specificity

Monoclonal Anti-Calponin specifically recognizes smooth muscle Calponin.

Immunogen

Synthetic peptide of the human p62c-myc protein.

Application

Anti-c-Myc-Peroxidase antibody, Mouse monoclonal may be used in immunoblotting and immunohistology.

Biochem/physiol Actions

c-Myc is a transcription factor involved in regulation of cell growth, proliferation, differentiation and apoptosis. It is implicated in DNA replication, RNA splicing or transcription. Increased expression of the gene is associated with the development of various types of human cancers.

Physical form

Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline

Storage and Stability

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable