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SAB4200171

Sigma-Aldrich

Anti-NEZHA (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-KIAA1543, Anti-calmodulin-regulated spectrin-associated protein 3

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~150 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5.0-10 μg using lysates of HEK- 293T cells overexpressing human NEZHA.
indirect immunofluorescence: 2.5-5.0 μg/mL using MDCK fixed cells.
western blot: 0.5-1 μg/mL using lysates of HEK- 293T cells overexpressing human NEZHA.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Specificity

Anti-NEZHA (C-terminal) recognizes human NEZHA.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Andrew Muroyama et al.
Molecular biology of the cell, 29(13), 1533-1541 (2018-05-10)
In most differentiated cells, microtubules reorganize into noncentrosomal arrays that are cell-type specific. In the columnar absorptive enterocytes of the intestine, microtubules form polarized apical-basal arrays that have been proposed to play multiple roles. However, in vivo testing of these

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