S5768

Monoclonal Anti-Synaptophysin antibody produced in mouse

clone SVP-38, ascites fluid

• Synonym(s): Synaptophysin Antibody, Synaptophysin Antibody - Monoclonal Anti-Synaptophysin antibody produced in mouse
• MDL number: MFCD00164741
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: SVP-38, monoclonal
• mol wt: antigen 38 kDa
• contains: 15 mM sodium azide
• species reactivity: pig, rat, human, guinea pig
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): 1:200 using rat cerebellum; indirect ELISA: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P08247
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SYP(6855) ; rat ... Syp(24804)

Description

General description

Synaptophysin (SYP) is an integral membrane glycoprotein of 38,000 Da and it has four membrane-spanning domains. Synaptophysin is localized to neurons, neuromuscular junctions, paraganglia cells, hypophysis, pancreatic islet cells, and adrenal cells. SYP gene is mapped to human chromosome Xp11.23. Monoclonal Anti-Synaptophysin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Labels synaptophysin in neurons, neuromuscular junctions, benign and malignant neural tumors, paraganglia cells, hypophysis, pancreatic islet cells, and adrenal cells.

Immunogen

Rat retina synaptosome.

Application

Monoclonal Anti-Synaptophysin antibody has been used in western blotting.

Biochem/physiol Actions

Synaptophysin participates in calcium dependent exocytosis. The antibody detects synaptophysin in many types of benign and malignant neural and epithelial neuroendocrine neoplasms. It is widely used as a potential marker to study the circulation of synapses in the brain and to discover the basic features of the life cycle of synaptic vesicles (SVs).

Physical form

Monoclonal Anti-Synaptophysin is provided as ascites fluid containing 0.1% sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable