The Assay/Sample Diluent Buffer (Item E2) should be diluted 5-fold. This 1X Assay/Sample Diluent Buffer is used to dilute serum, plasma, or medium samples a recommended 2 fold. Please note that the levels of IL-6 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator.
RAB0308
Mouse IL-6 ELISA Kit
for serum, plasma and cell culture supernatant
Synonym(s):
B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IL-6, Interferon beta-2
Sign Into View Organizational & Contract Pricing
Select a Size
1 KIT
MXP 12,604.00
MXP 12,604.00
In StockDetails
All Photos(2)
Select a Size
Change View
1 KIT
MXP 12,604.00
About This Item
UNSPSC Code:
41116158
NACRES:
NA.84
MXP 12,604.00
In StockDetails
Recommended Products
species reactivity
mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type plasma
sample type cell culture supernatant(s)
sample type serum
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 0.82-600 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Il6(24498)
General description
The Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-6 in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Mouse IL-6
Application
Biochem/physiol Actions
IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism.[4]
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
recommended
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
The biology of interleukin-6.
Kishimoto T
Blood, 74(1), 1-10 (1989)
Effect of HI-6 on cytokines production after immunity stimulation by keyhole limpet hemocyanin in a mouse model.
Pohanka M
Neuro Endocrinology Letters, 35, 155-157 (2014)
Cytocompatible Anti-microbial Dressings of Syzygium cumini Cellulose Nanocrystals Decorated with Silver Nanoparticles Accelerate Acute and Diabetic Wound Healing.
Singla R
Scientific Reports, 7(1), 10457-10457 (2017)
Laura N Castellani et al.
Scientific reports, 8(1), 772-772 (2018-01-18)
Olanzapine is a widely prescribed antipsychotic drug. While effective in reducing psychoses, treatment with olanzapine causes rapid increases in blood glucose. We wanted to determine if a single bout of exercise, immediately prior to treatment, would attenuate the olanzapine-induced rise
Rubbel Singla et al.
Scientific reports, 7(1), 10457-10457 (2017-09-07)
The ever increasing incidences of non-healing skin wounds have paved way for many efforts on the convoluted process of wound healing. Unfortunately, the lack of relevance and success of modern wound dressings in healing of acute and diabetic wounds still
-
Hello. How much should I dilute tissue culture media for the assay? 2-fold? Thanks.
1 answer-
Helpful?
-
-
マウスのパイエル板をホモジナイズした上清で測定は可能ですか？
1 answer-
This product is suitable with serum, plasma, and cell culture supernatant. However, it has not been determined if this product can measure the supernatant obtained by homogenizing mouse Peyer's patches. This application will need to be validated by the end-user.
While the requested ELISA kit nor any of the offered normal ELISA kits are neither designed nor validated with cell/tissue lysate samples, our kits are very accommodating to many different sample types so lysates will most likely work. If it is decided to test these kits with cell/tissue lysate samples, it is recommended to dilute the samples at least 5-fold with Assay Diluent to minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined by the experimenter empirically.
So for the original lysate, in general it is recommended to shoot for at least 1 mg/ml protein, preferably more, to achieve 50-500 ug/ml after dilution. Again though, this range should just be used as a starting point and the optimal concentration would need to be determined empirically.
Below are some general guidelines for doing so along with some basic tips for sample preparation.
Tips on Sample preparation
In brief, a lysis buffer must meet the following specifications:
A) has relatively low salt content (700 mM or less)
B) does not contain sodium azide
C) does not contain >0.1% SDS
D) does not contain >10 mM reducing agents (beta-mercaptoethanol or dithiothreitol)
This would include any buffers used for immunoprecipitations, including RIPA buffer.Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
