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biological source
rat heart (Sprague Dawley, normal adult)
Quality Level
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 42
morphology
fibroblast
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cardiovascular diseases
shipped in
dry ice
storage temp.
−196°C
General description
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Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. They are known to play key roles in the development of cardiac disease and hypertrophy. Rat Cardiac Fibroblasts provide an excellent model system to study many aspects of heart function and pathophysiology, including the regulation of cell growth, survival, inflammation, hormone secretion and metabolism.
Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. They are known to play key roles in the development of cardiac disease and hypertrophy. Rat Cardiac Fibroblasts provide an excellent model system to study many aspects of heart function and pathophysiology, including the regulation of cell growth, survival, inflammation, hormone secretion and metabolism.
Cell Line Origin
Heart
Application
human heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokines
Components
Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
Preparation Note
- 2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 8 population doublings
Subculture Routine
Please refer to the RCF Culture Protocol.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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