Skip to Content
Merck
All Photos(6)

Key Documents

View All Documentation

N2127

Sigma-Aldrich

4-Nitrophenyl α-D-mannopyranoside

α-mannosidase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

4-Nitrophenyl a-D-mannopyranoside, 4-Nitrophenyl alpha-D-mannopyranoside, pNP-alpha-D-Man, pNPalphaMan

Sign Into View Organizational & Contract Pricing

Select a Size

25 MG
MXP 1,802.00
100 MG
MXP 2,191.00
250 MG
MXP 4,216.00
500 MG
MXP 9,058.00
1 G
MXP 13,587.00
5 G
MXP 52,177.00

MXP 1,802.00

Check Cart for Availability
All Photos(6)

Select a Size

Change View
25 MG
MXP 1,802.00
100 MG
MXP 2,191.00
250 MG
MXP 4,216.00
500 MG
MXP 9,058.00
1 G
MXP 13,587.00
5 G
MXP 52,177.00

About This Item

Empirical Formula (Hill Notation):
C12H15NO8
CAS Number:
10357-27-4
Molecular Weight:
301.25
Beilstein:
92210
EC Number:
233-776-6
MDL number:
MFCD00066002
UNSPSC Code:
12352204
PubChem Substance ID:
24897554
NACRES:
NA.32

MXP 1,802.00

Check Cart for Availability

Product Name

4-Nitrophenyl α-D-mannopyranoside, α-mannosidase substrate

Quality Level

300

Assay

≥98% (TLC)

form

powder

solubility

DMF: 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](Oc2ccc(cc2)[N+]([O-])=O)[C@@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11+,12+/m1/s1

InChI key

IFBHRQDFSNCLOZ-GCHJQGSQSA-N

Looking for similar products? Visit Product Comparison Guide

General description

4-Nitrophenyl α-D-mannopyranoside is a chromogenic substrate for α-mannosidase.[1]

Application

4-Nitrophenyl α-D-mannopyranoside has been used as a substrate for α-mannosidase[2] in hydrolase activity assay.[3]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000423317
0000423316
0000423313
0000423313
0000423316

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Daniel J Coleman et al.
Analytical biochemistry, 399(1), 7-12 (2009-12-23)
A simple and reliable continuous assay for measurement of alpha-mannosidase activity is described and demonstrated for analysis with two recombinant human enzymes using the new substrate resorufin alpha-d-mannopyranoside (Res-Man). The product of enzyme reaction, resorufin, exhibits fluorescence emission at 585
P N Kanellopoulos et al.
Journal of structural biology, 116(3), 345-355 (1996-05-01)
Concanavalin A (Con A) is the best-known plant lectin and has important in vitro biological activities arising from its specific saccharide-binding ability. Its exact biological role still remains unknown. The complexes of Con A with 4'-nitrophenyl-alpha-D-mannopyranoside (alpha-PNM) and 4'-nitrophenyl-alpha-D-glucopyranoside (alpha-PNG)
S J Hamodrakas et al.
International journal of biological macromolecules, 11(1), 17-22 (1989-02-01)
Molecular models for the complex formed between the lectin concanavalin A (Con A) and the saccharide derivative 4'-nitrophenyl-alpha-D-mannopyranoside (alpha-PNM) are presented, combining evidence from 1H-n.m.r. measurements, semi-empirical energy calculations and interactive graphics modelling. The models are in good agreement with
S Jelinek-Kelly et al.
The Journal of biological chemistry, 260(4), 2253-2257 (1985-02-25)
Fractionation of a crude extract from Saccharomyces cerevisiae X-2180 on Sepharose 6B in the presence of 0.5% Triton X-100 resolves two enzyme fractions containing alpha-mannosidase activity. Fraction I which is excluded from the gel contains alpha-mannosidase activity toward both p-nitrophenyl-alpha-D-mannopyranoside
Y Ohyama et al.
The Journal of biological chemistry, 260(11), 6882-6887 (1985-06-10)
The interactions of Sepharose 4B-immobilized concanavalin A (ConA) with 10 glycoasparagines derived from ovalbumin were investigated quantitatively by frontal affinity chromatography. In this method, a carbohydrate solution is applied continuously to a ConA-Sepharose column and the retardation of the elution
View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service