MAK333
Citrate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Citric Acid Test Kit
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About This Item
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
input
culture(s)
plasma
tissue
serum
urine
detection method
colorimetric
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
Application
The Citrate Assay Kit may be used for:
- Cancer Research
- Drug Discovery
- Metabolic Disorders Research
Features and Benefits
Efficient and Precise: Detect Citrate levels ranging from 4 to 400 μM (colorimetric) and 0.5 to 40 μM (fluorimetric) in a 15 minute room temperature reaction, providing both convenience and sensitivity.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Suitability
Suitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)
Principle
In this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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