Skip to Content
Merck
All Photos(1)

Documents

MAK265

Sigma-Aldrich

Fructose Colorimetric/Fluorometric Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Fructose Detection Kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

Fructose is a monosaccharide naturally found in honey and fruits. Several studies have linked fructose to the development of the metabolic syndrome particularly through promoting insulin resistance in the liver. It has also been implicated in the obesity epidemic. Studies in animals have revealed high fructose intake during pregnancy and lactation may lead to metabolic dysfunctions in the mother and the newborn. Fructose measurements can provide useful insights into metabolic and biochemical functions. Excessive uptake of fructose is implicated in obesity in children. Fructose induced hyperuricemia, reduces nitric oxide bioavailability for insulin and thus it is implicated in diabetes. Fructose might be implicated in cardiovascular disease, as it is the only sugar that raises uric acid production in the body. High fructose diet is known to induce hypertension.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for detecting fructose in cell and tissue culture supernatants. Note: Not suitable for urine, plasma, or serum.

Principle

In this assay kit, fructose is converted to -glucose resulting in a colorimetric (570 nm)/fluorometric (ex = 535 nm/em = 587 nm) product proportional to fructose present. This kit has a linear range of detection between 0.2–1.0 nmole fructose for the fluorometric assay and 2–10 nmole fructose for the colorimetric assay.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Potential role of sugar (fructose) in the epidemic of hypertension, obesity and the metabolic syndrome, diabetes, kidney disease, and cardiovascular disease.
Johnson R J, et al.
American Journal of Clinical Nutrition, 86(4), 899-906 (2007)
A causal role for uric acid in fructose-induced metabolic syndrome.
Nakagawa T, et al.
American Journal of Physiology: Renal Physiology, 290(3), F625-F631 (2006)
Nattawan Suwannakul et al.
Genes & diseases, 9(6), 1727-1741 (2022-09-27)
Alterations in cellular metabolism may contribute to tumor proliferation and survival. Upregulation of the facilitative glucose transporter (GLUT) plays a key role in promoting cancer. GLUT5 mediates modulation of fructose utilization, and its overexpression has been associated with poor prognosis

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service