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L7026

Sigma-Aldrich

Lithium chloride solution

8 M, for molecular biology, ≥99%

Synonym(s):

LiCl

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About This Item

Linear Formula:
LiCl
CAS Number:
7447-41-8
Molecular Weight:
42.39
MDL number:
MFCD00011078
UNSPSC Code:
12352302
PubChem Substance ID:
24896448
NACRES:
NA.31

grade

for molecular biology

Quality Level

200

Assay

≥99%

concentration

8 M

anion traces

sulfate (SO42-): ≤0.01%

cation traces

Ba: ≤0.003%
Fe: ≤0.001%
K: ≤0.01%
Na: ≤0.20%
heavy metals (as Pb): ≤0.002%

foreign activity

DNase, RNase, protease, none detected

SMILES string

[Li+].[Cl-]

InChI

1S/ClH.Li/h1H;/q;+1/p-1

InChI key

KWGKDLIKAYFUFQ-UHFFFAOYSA-M

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Application

Lithium chloride solution has been used:
  • to obtain LiClPAM3 DCs form of dendritic cell
  • in LiCl buffer preparation for immunoprecipitation
  • in the preparation of wash buffer
  • in the preparation of radioimmunoprecipitation assay (RIPA) wash buffer

May be used to selectively precipitate RNA.

Biochem/physiol Actions

Lithium chloride has the ability to block glycogen synthase kinase (GSK). It may also possess anti-inflammatory effects at low and non-toxic concentrations.

Preparation Note

Prepared with 18 megaohm water
0.2 μm filtered

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302,H315,H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
Cecere G and Grishok A
Nature Structural and Molecular Biology (2014)
mTOR and neuroinflammation
Molecules to Medicine with mTOR, 317-329 (2016)
Micro-chromatin Immunoprecipation (muChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
Gillotin S and Guillemot F
Bio-protocol, 6(12), e0159986-e0159986 (2016)
Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype
Kouwenberg M, et al.
PLoS ONE, 11(8), e0159986-e0159986 (2016)
Tong Ihn Lee et al.
Nature protocols, 1(2), 729-748 (2007-04-05)
Genome-wide location analysis, also known as ChIP-Chip, combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in living cells. Protein-DNA interactions are captured in vivo by chemical crosslinking. Cell lysis, DNA fragmentation and immunoaffinity purification of
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