L4543

Anti-L1CAM antibody, Mouse monoclonal

clone UJ127.11, purified from hybridoma cell culture

• Synonym(s): L1CAM Antibody - Anti-L1CAM antibody, Mouse monoclonal, L1Cam Antibody, Anti-CAML1, Anti-L1 Cell Adhesion Molecule, Anti-MIC5, Anti-Neural Cell Adhesion Molecule L1
• MDL number: MFCD01864727
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: UJ127.11, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 220-240 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using cell extracts of G361 cells (human melanoma)
• isotype: IgG1
• UniProt accession no.: P32004
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... L1CAM(3897)

Description

General description

Anti-L1CAM antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma UJ127.11 produced by the fusion of mouse myeloma cells (P3- X63-Ag8-653 cells) and splenocytes from mice immunized with human fetal brain. L1 cell adhesion molecule (L1CAM) protein belongs to the family of cell adhesion molecules (CAM).

The gene L1CAM (L1 cell adhesion molecule) encodes a member of the immunoglobulin supergene family. The encoded protein contains a cytoplasmic intracellular domain, one single-pass transmembrane region and an extracellular domain containing six immunoglobulin- (Ig) and five fibronectin (Fn) III-like motifs. The gene is mapped to human chromosome Xq28. It contains 29 exons that encode a protein of 1257 amino acids containing a 19 amino acid signal peptide and a functional L1 protein of 1238 amino acids. It is mainly expressed in the nervous system.

Immunogen

L1CAM from human fetal brain.

Application

Anti-L1CAM antibody has been used in immunohistochemistry.

Anti-L1CAM antibody, Mouse monoclonal has been used for:

• immunohistochemistry
• immunoprecipitation
• immunoblotting
• immunocytochemistry
• flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

L1 cell adhesion molecule (L1CAM) is critical for central nervous system (CNS) development in humans. This protein is responsible for many activities among them: neurite outgrowth, adhesion, fasciculation and myelination. These activities are mediated by the interaction of L1CAM with various members of the CAM protein family, extracellular matrix molecules and signaling receptors, through interactions that involve the extracellular domain of L1CAM. Mutations in the L1CAM gene may be linked to several brain disorders such as type 1 X-linked spastic paraplegia (SPG1), MASA syndrome and ACC (agenesis of corpus callosum).

The gene L1CAM (L1 cell adhesion molecule) encodes a glycoprotein that functions in axon growth during development and axon bundling. It interacts with Schwann cells and axons, and mediates neuronal cell migration, and neuronal cell survival. Overexpression of this protein is associated with tumor progression. It has been found to mediate therapeutic resistance in ovarian and pancreatic cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable