J4750

Anti-JNK, Activated (Diphosphorylated JNK) antibody, Mouse monoclonal

clone JNK-PT48, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: JNK-PT48, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 46 kDa; antigen 54 kDa
• species reactivity: mouse, human, rat
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 4-20 μg/mL using extract of human Jurkat cells activated with anisomycin
• isotype: IgG1
• UniProt accession no.: P45983
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAPK8(5599) ; mouse ... Mapk8(26419) ; rat ... Mapk8(116554)

Description

General description

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) (mouse IgG1 isotype) is derived from the JNK-PT48 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the phosphorylated form of JNK activation loop, conjugated to KLH. JNK (c-Jun N terminal protein kinase) belongs to the mitogen-activated protein kinase (MAPK) superfamily of enzymes. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residue determines the specificity of activation of the MAPKs and is proline for JNK. JNK1-3 of molecular weights 46, 54, and 52 kDa respectively.

Specificity

The antibody reacts specifically with the activated diphosphorylated form of JNK. The epitope recognized by the antibody contains the regulatory site of JNK (Thr¹⁸³ and Tyr¹⁸⁵). Weak cross-reaction is observed with the monophosphorylated threonine peptide, but not with the non-phosphorylated or monophosphorylated tyrosine peptides of JNK. It does not recognize any form of p38-MAPK or ERK-MAPK.

Immunogen

synthetic peptide sequence containing amino acids pTPpYVVTRYYR, corresponding to the phosphorylated form of JNK-activation loop.

Application

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody has been used in immunoblotting, enzyme linked immunosorbent assay (ELISA), immunocytochemistry, western blotting, immunohistochemistry.

Biochem/physiol Actions

c-Jun N terminal protein kinase (JNK) is mostly stress-responsive kinase and have been implicated in cell death in several cellular systems. The JNK cascade is triggered by small GTPases that are Rac and Cell division control protein 42 (CDC42). After activation, JNKs translocate to the nucleus where they physically associate with and activate their target transcription factors (cJun, AMP-dependent transcription factor (ATF) etc.).

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable