I3909

Ionomycin calcium salt

Ready Made Solution, from Streptomyces conglobatus, 1 mM in DMSO

• Synonym(s): Calcium Ionophore Ionomycin
• Empirical Formula (Hill Notation): C₄₁H₇₀CaO₉
• Molecular Weight: 747.07
• EC Number: 200-664-3
• MDL number: MFCD00083634
• UNSPSC Code: 51102829
• PubChem Substance ID: 329815372
• NACRES: NA.85

Properties

• biological source: Streptomyces conglobatus
• Quality Level: 300
• Assay: ≥98% (HPLC)
• shelf life: 2 yr
• storage condition: (Tightly closed. )
• concentration: 1 mM in DMSO
• color: colorless
• mp: 18.4 °C
• solubility: DMSO: 1 mM
• antibiotic activity spectrum: Gram-positive bacteria
• Mode of action: cell membrane | interferes
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: C[C@@H]1CCC(=O)O[Ca]OC(=CC(=O)[C@@H](C)C[C@@H](C)C1)[C@H](C)C[C@H](C)C\C=C\[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]2CC[C@](C)(O2)[C@H]3CC[C@](C)(O3)[C@@H](C)O
• InChI: 1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1
• InChI key: WKRWUYKLUMMAKG-WYGBAUISSA-L

Description

General description

Ionomycin is a calcium ionophore commonly derived from produced by the bacterium Streptomyces conglobatus, which means that it helps to transport calcium ions across cell membranes. It is a powerful tool for studying calcium signaling and its role in various biological processes, including cell activation, secretion, proliferation, and apoptosis.

Ionomycin is commonly used in cell biology experiments to induce a rapid and controlled increase in intracellular calcium levels. This allows to study the downstream effects of calcium signaling pathways, such as the activation of enzymes, the release of hormones, and the changes in gene expression.Ionomycin is also used to investigate the role of calcium in specific cellular processes, such as cell death, immune cell activation, and muscle contraction. For example, ionomycin can be used to induce apoptosis in cancer cells, study the activation of T cells, and investigate the role of calcium in muscle contraction.

Application

Ionomycin finds application in:

• Study of calcium signaling and its role in various biological processes
• Investigation of intracellular calcium levels, calcium-dependent enzymes, and cellular responses triggered by calcium influx
• Induction of a rapid and controlled increase in intracellular calcium levels in cell biology experiments
• Elucidation of specific cellular mechanisms and signaling pathways in combination with other substances, such as fluorescent calcium indicators or protein kinase activators

Ionomycin has been used:

• to induce central demyelination[1]
• inhibit adrenal bovine TREK-1 channels[2]
• to regulate cell division of mature human B cells[3]

Biochem/physiol Actions

Ca²⁺ ionophore that is more effective than A23187 as a mobile ion carrier for Ca²⁺.

Ionomycin is an ionophoric antibiotic that binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations.

Mode of Action: Facilitates the transfer of calcium ions (Ca2+) across biological membranes

Antimicrobial Spectrum: Active against Gram-Positive bacteria

Ionomycin is an ionophoric antibiotic which binds Pb²⁺ and Ca²⁺, and serves as an effective mobile carrier of both cations. Ionomycin is more effective than A23187 as a mobile ion carrier for Ca²⁺. It is non-fluorescent. It is used to study Ca²⁺ transport across biological membranes and induces apoptotic degeneration of embryonic cortical neurons.

Features and Benefits

• Ready available solution reduce the need for preparation time
• Efficient choice for studying calcium signaling and its role in various biological processes
• Allows for rapid and controlled manipulation of intracellular calcium levels
• Versatile and widely used in cell biology research

Other Notes

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Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves