H0527

HEPES potassium salt

≥99.5% (titration)

• Synonym(s): Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-monopotassium salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) potassium salt
• Empirical Formula (Hill Notation): C₈H₁₇KN₂O₄S
• CAS Number: 82207-62-3
• Molecular Weight: 276.39
• MDL number: MFCD00043438
• UNSPSC Code: 12161700
• PubChem Substance ID: 24895405
• NACRES: NA.25

Properties

• Assay: ≥99.5% (titration)
• form: powder
• useful pH range: 6.8-8.2
• pKa: 7.5
• solubility: water: 0.5 g/mL, clear, colorless
• SMILES string: [K+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.K/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• InChI key: KWURIFKZTJGMFD-UHFFFAOYSA-M

Description

General description

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) is a widely used Good’s buffer in biochemical and biological research applications. As a zwitterionic buffer, it stabilizes the pH of the solution even when small amounts of acid or base are added.

Application

HEPES potassium salt has been used as a buffering agent in nanopore fabrication and electrolyte preparations. It has also been used as a component of cell lysis buffer.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves