GE27-9263-01
Ready-to-go™ T-Primed First-Strand Kit
Cytiva 27-9263-01, sufficient for 55 reactions
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About This Item
usage
sufficient for 55 reactions
feature
hotstart: no
manufacturer/tradename
Cytiva 27-9263-01
color
colorless
General description
The kit provides 50 single-dose, ambient-temperature-stable, first-strand cDNA synthesis reactions, each containing NotI-(dT)18 Primer, Cloned FPLCpure M-MuLV Reverse Transcriptase, RNase Inhibitor, and nucleotides.
Application
The kit provides 50 single-dose, ambient-temperature-stable, first-strand cDNA synthesis reactions, each containing NotI-(dT)18 Primer, Cloned FPLCpure M-MuLV Reverse Transcriptase, RNase Inhibitor, and nucleotides. (A reaction mixture containing rabbit globin mRNA and specific globin primers is included as a control).
Features and Benefits
- For synthesis of full-length first-strand cDNA templates from polyadenylated RNA for use in RT-PCR.
- Preformulated, ambient-temperature-stable, single-dose glassified reaction mixes minimize pipetting errors, avoid potential cross-contamination of subsequent PCR products, and ensure optimal performance from each reaction.
- Predispensed reaction mixes are highly suitable for research applications which use PCR to detect and quantify eukaryotic RNA species isolated from a variety of cell sources and tissue samples.
- Reaction mixes include NotI-(dT)18 primer in sufficient quantity to serve as a downstream PCR primer for performing 3′-RACE. Final reaction volume is 33 μL once reconstituted.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Ready-To-Go is a trademark of Cytiva
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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