G75120

Sephadex^® G-75

Medium

• Synonym(s): Gel filtration media, Gel filtration resin
• CAS Number: 37224-29-6
• MDL number: MFCD00132772
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• Quality Level: 200
• technique(s): buffer exchange: suitable
• matrix active group: phase
• swelling: 1 g swells to 12-15 mL gel
• bead diameter: 40-120 μm
• application(s): life science and biopharma
• compatibility: Cytiva

Description

General description

Sephadex G-75 is widely used for desalting and buffer exchange. This gel filtration resin is created through the crosslinking of dextran with epichlorohydrin. Sephadex varies in its cross-linking level across different types, influencing its swelling capacity and molecular fractionation range.

Sephadex^® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex^® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.

Application

Sephadex^® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.[1][2]

Sephadex^® G-75 has been used to separate the products from the pepsin digest by gel filtration chromatography[3] and to desalt low density lipoproteins (LDL) by gel filtration.[4]

Features and Benefits

• Enables rapid desalting, contaminant removal, and buffer exchange in a single step.
• Ideal for buffer exchange and cleanup of large biomolecules with a molecular weight exceeding 80,000.
• Features the highest exclusion limit of 80,000 within the Sephadex G-range.
• Classic gel filtration resin.

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves