F7252

Fast Green FCF

Dye content ≥85 %, powder

• Synonym(s): Food green 3
• Linear Formula: C₃₇H₃₄N₂O₁₀S₃Na₂
• CAS Number: 2353-45-9
• Molecular Weight: 808.85
• Colour Index Number: 42053
• Beilstein: 5718212
• EC Number: 219-091-5
• MDL number: MFCD00013053
• UNSPSC Code: 12352204
• PubChem Substance ID: 57654190
• NACRES: NA.83

Properties

• Product Name: Fast Green FCF, Dye content ≥85 %
• form: powder
• Quality Level: 200
• composition: Dye content, ≥85%
• mp: 290 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL, clear
• SMILES string: [Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
• InChI: 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
• InChI key: RZSYLLSAWYUBPE-UHFFFAOYSA-L

Description

General description

Fast Green FCF is one of the triarylmethane dyes,[1] that is biocompatible.[2] This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics. [2]

Application

Fast Green FCF has been used for in staining of menisci from rabbit[3] and of osteochondral tissues.[4]

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Biochem/physiol Actions

Fast Green FCF can be considered as a safe stain to detect tumor.[1] In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.[2]

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H335,H341
• Precautionary Statements: P202 - P261 - P271 - P280 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Muta. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): 352.4 °F - closed cup
• Flash Point(C): 178 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves