E8027

EphA2/Fc Chimera from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(s): Eck, Myk2, Sek
• MDL number: MFCD01867520
• UNSPSC Code: 51111800

Properties

• biological source: mouse
• recombinant: expressed in NSO cells
• Assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.078-5 ng/mL
• mol wt: monomer calculated mol wt 84 kDa; ~105 kDa by SDS-PAGE (reducing)
• packaging: pkg of 200 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): ligand binding assay: suitable
• impurities: endotoxin, tested
• UniProt accession no.: Q03145
• storage temp.: −20°C
• Gene Information: mouse ... Epha2(13836)

Description

General description

EPHA2 (ephrin receptor A2) belongs to the Eph family of receptor tyrosine kinases. It is a type I transmembrane protein, and its exoplasmic domain consists of a ligand binding domain in its N-terminal, a cysteine-rich domain, and two fibronectin type III repeats. Its cytoplasmic domain contains a juxtamembrane region, a tyrosine kinase domain, a SAM (sterile-a-motif) motif, and a PDZ-binding domain.

Biochem/physiol Actions

EPHA2 binds to the ephrin-A ligand and has diverse cellular function. EPHA2 has been shown to be an oncoprotein of importance in a range of cancers. EPHA2 is overexpressed in several human cancer types and promotes malignancy through a mechanism involving RhoA-dependent destabilization of adherens junctions. EPHA2 overexpression induces a FAK-dependent increase in MMP-2 expression and invasiveness and this process that can be reversed by ligation of EPHA2.

Member of the Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4, and ephrin-A5; involved in pattern formation and morphogenesis. EphA2 is expressed on endothelial cells and in tumor xenographs and may regulate tumor neovascularization.

Other Notes

Extracellular domain of mouse EphA2 (amino acids 1-535) fused by means of a polypeptide linker to the Fc region of human IgG1 that is histidine-tagged at the C-terminus.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.0.

Analysis Note

The biological activity is measured by its ability to bind recombinant mouse ephrin-A1/Fc in an ELISA assay.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves