D6293

pUC18 DNA Hae III Digest

for DNA electrophoresis

• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• form: liquid
• usage: 200 uses (0.1 mg size); 40 uses (20 μg size)
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Description

General description

Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.

Components

Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 11 fragments, 11–587 bp

For optimal resolution, the recommended agarose gel concentration is 2.5%.

Preparation Note

Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable