Product No. D0564 is the wild-type toxin and possesses enzymatic activity. The Glu52 diptheria toxin, also known as CRM197, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity. More information on the mutant can be found in the following reference: Uchida, T., Pappenheimer, Jr., A.M. and Harper, A.A., J. Biol. Chem., 248, 3851-3854 (1973).
D2189
Recombinant CRM197 expressed in Pseudomonas fluorescens, [Glu52]- Diphtheria toxin
lyophilized powder
Synonym(s):
[Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, CRM 197
About This Item
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Packaging
Physical form
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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What kind of mutant, is Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, and how is this product different from Product No. D0564?
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What is the molecular weight of Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?
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On reducing SDS gels, this protein migrates as a single band of an approximate molecular weight of 63,000 Daltons.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I reconstitute Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?
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The vial can be reconstituted with 1.0 mL of sterile water. Each vial contains 1 mg of Diphtheria toxin CRM mutant, sodium phosphate buffer, and lactose. It can sometimes be hard to get the product into solution due to the lactose used as a stabilizer. The supplier suggests slight warming (to at least room temperature or 30 °C, but not more than 37 °C) followed by a brief vortex to break up the filaments.
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