Derived from the ethylnitrosourea-induced tumour line D6 of the rat peripheral nervous system. Cells will de-differentiate after 10 passages. Read references prior to use.
Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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