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RENT tsS1.1

92092515, rat endometrium, Cuboidal

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About This Item

UNSPSC Code:
41106514

product name

RENT tsS1.1, 92092515

biological source

rat endometrium

growth mode

Adherent

karyotype

Not specified

morphology

Cuboidal

products

Not specified

receptors

Glucocorticoid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

Cell Line Description

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43 markers. The transforming agent is a ts mutant of SV 40 T-antigen (tsA58).

Culture Medium

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

Other Notes

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