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biological source
Human x Mouse Hybridoma hybrid
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse x Human hybrid myeloma
Cell Line Description
Derived by a fusion of NS-1 - Ag4 myeloma cells with cells from a human nodular lymphoma. The cells are HPRT- and do not secrete immunoglobulin. They are used as a fusion partner for human B lymphocytes.
Application
Fusion partner and hybridisation studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 1 x 105 to 1 x 106 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
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