OX-41

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 88051304, Reactivity: Anti-Macrophage subset, rat

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 88051304-DNA-5UG; pkg of vial of cells 88051304-1VL
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS0/U
• Reactivity: Anti-Macrophage subset, rat
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse NS0/U x Mouse BALB/c spleen

Cell Line Description

The antibody recognises a rat macrophage cell surface antigen and labels all peritoneal and 90% of alveolar macrophages. Splenic red pulp macrophages are antigen +ve, marginal zone macrophages antigen -ve. The antibody also reacts with the 120kDa antigen on granulocytes and dendritic cells and recognises rat SIRP (personal communication).

Immunogen

Resident peritoneal cells from (PVG-RT1c x PVG RT1u)F1 and (PVG-RT1c x DA-RT1a)F1 rats

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.