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biological source
Mouse x Rat Heterohybridoma
ImmunoDon
LOU/Ws1 rat spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-κ-Chain, mouse
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse P3X63Ag8.653 x Rat LOU/Ws1 spleen
Cell Line Description
The antibody is directed against murine kappa chain.
Immunogen
Purified murine immunoglobulin
Application
The isotype of the monoclonal antibody produced by this cell line is rat IgG1, kappa.
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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